Involvement of Arabidopsis HIT1 in the preservation of endomembrane integrity under stress conditions and the mapping of Arabidopsis ret8 locus

• Main Authors: Yu-Shan Fan, 范育珊
• Other Authors: Shaw-Jye Wu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33571569312159525492

碩士 === 國立中央大學 === 生命科學研究所 === 100 === Arabidopsis hit1-1 is a heat-intolerant mutant. The HIT1 gene was found to encode a protein with homology to yeast Vps53p, which is a subunit of the Golgi-associated retrograde protein (GARP) complex. The tethering complex includes Vps51p, Vps52p and Vps54p, that is involved in retrograde trafficking of vesicles to the Golgi. hit1-1 is hypersensitivity to heat stress may be due to the loss plasma membrane integrity caused by ineffective vesicle recycling. When a tethering factor lost the function not only plasma membrane but also endomembrane may be affected. To test this hypothesis, various transgenic Arabidopsis lines expressing YFP-tagged reporter of the Golgi, endoplasmic reticulum (ER), vacuole and mCherry-tagged reporter of plasma membrane (PM) were generated. These transgenic plants allow us to monitor and compare morphological changes of various endomembrane compartments in both wild type and hit1-1 mutant. Ethylene is a gaseous phytohormone. It is involved in regulation of many aspects of plant growth and development. To gain more understanding about ethylene biology, we used small molecules (hit compounds) to screen Arabidopsis mutants, named revert to etol (ret), with restoration of the ethylene overproducer 1 (eto1) phenotype (triple response) in the presence of hit compounds. One of the ret mutants, ret8, which was identified from Arabidopsis after ethyl-methane sulfonate (EMS) –mutagenesis. To identify the gene underlying the ret8 phenotype, map-based cloning of RET8 gene is being conducted.