The Value of Using Neutral DNA Probes on Biosensor Comparing with Regular DNA Probes Depicted by SPRi & FET

• Main Authors: Hong-Cheng Chen, 陳泓成
• Other Authors: Wen-Yih Chen
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/07952703140628604953

碩士 === 國立中央大學 === 化學工程與材料工程研究所 === 100 === DNA biosensors based on sequence-specific DNA hybridization have been widely used in clinical diagnostics, forensic sciences and biomedical research. In recent years intensive efforts have been focused on the development of ultrasensitive DNA biosensors capable of quantitative gene expression analysis. A large number of detection techniques and sensor surface preparation or probe immobilization methods have been developed in order to maximize the sensitivity and specificity. Nature of the sensing probe is one of the key elements generally dictating the performance of the sensor. Neutral DNA is an uncharged DNA analogue. It’s electrically neutral is due to the backbone modification. No electrostatic repulsive during the hybridization between neutral DNA probe and regular DNA from sample which makes the hybridization efficiency of the neutral DNA better than regular one. Here in the study, we adopted SPR and FET biosensor systems for validating the value of the neutral DNA on non-labeling gene chip applications.