Electrospinning of Chitosan/Alginate Composite Nanofibrous Structures for Cell Adhesion and Proliferation

• Main Authors: Hsing-ning Yu, 余興寧
• Other Authors: Wei-wen Hu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/39069674935200172157

碩士 === 國立中央大學 === 化學工程與材料工程研究所 === 100 === To custom-tailor biomaterial surfaces for tissue engineering application, we developed chitosan/alginate composite surfaces using electrospinning technique. Firstly, chitosan and alginte were solely electrospun, respectively. Poly (ethylene oxide) (PEO) was added to both chitosan and alginte solutions, and the effect of polymer composition on fibrous morphology was studied through scanning electrical microscopy (SEM). In addition, viscosities of polymer solution were also analyzed, demonstrating that higher concentrations of polymer caused higher viscosities, which directly determined the morphology of nanofibrous structure. Then, the relationship between flow rates and fiber deposition was also studied. By controlling the flow rates of chitosan/PEO and alginate/PEO solution separately, these nanofibers were able to be spun simultaneously with different deposition ratios. Finally, the composite nanofibrous surfaces were applied for cell culture. Because cell morphologies were different in chitosan and alginate nanofibrous surfaces, we may regulate cell morphologies by controlling the ratios of nanofibers, About biocompatibility, it was found that nanofibers can improve the cell viability over chitosan and alginate films. In addition, composite nanofibers even demonstrated greater biocompatibility than that of chitosan and alginate fibers. These results suggested that the ratio of nanofiber can be finely controlled through simultaneous electrospinning technique, and we may manipulate composite surface properties, which should be beneficial to biomaterial application.