The synthesis, protonation constants and stability constants of dinuclear macrocyclic ligands, MB-DO2A dimer , Methyl DO2A dimer and DO2A Dimer.

• Main Authors: Chin, Kuan-Hao, 金觀豪
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/92130859009796474612

碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 100 === We have been interested in the design, synthesis, and characterizations of trivalent lanthanide macrocyclic complexes for use as potential artificial nucleases due to their suitable properties such as high thermodynamic stability, low kinetic lability, high coordination number, and charge density (Lewis acidity). Our previous work showed that the monomeric complex, EuDO2A+, had higher BNPP hydrolysis rates as compared to other LnDO2A+ complexes, particularly at higher pH. However, at higher pH, the LnDO2A+ complexes tend to form oligomeric species and gradually lose their hydrolytic activities. To avoid the formation of the inactive oligomeric species, we have designed and synthesized a ligand capable of forming dinuclear lanthanide complexes based on DO2A, i.e. MB-DO2Adimer , Methyl DO2A Dimer and DO2A Dimer. In this articale, we report the details of the ligand synthesis and initicial characterizations of the ligand and its Metal-complexes. The result shows that the complexes solution of Ca2+/Eu3+-MB-DO2A Dimer becomes misty and precipitates form after pH 7.0. Methyl DO2A Dimer coordinated with Eu3+ also formed precipitates slightly, means both are not suitable for futher kinetic study. However, the complexes solution of Ca2+/ Eu3+-DO2A Dimer would not become misty, so we can expect the futher application of DO2A Dimer in the study of artificial nuclease.