Fabrication of ARROW-B SPR Biosensors and Detection of Protein Kinase A Activity

• Main Authors: Cheng, Jain-Wei, 程建瑋
• Other Authors: Huang, Yang-Tung
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/39824162305522829504

碩士 === 國立交通大學 === 電子研究所 === 100 === In this study, an antiresonant reflecting optical waveguide of type B (ARROW-B) surface plasmon resonance (SPR) biosensor operating in the aqueous environment with a circulating-flow system has been investigated. The ARROW-B SPR biosensor is proposed to provide a label-free, high-efficiency, and highly surface-sensitive platform to detect the bimolecular interactions. Modal characteristics of ARROW-B are analyzed with simulation and designed for obtaining optimum sensitivity in aqueous environment. The waveguides in the front and rear of the SPR sensing regions have a symmetric cladding structure to prevent from being affected by environmental changes, and the sensing region is configured with a liquid flow channel which assists bioagents in attaching to the gold surface for reaction. For the bioassay experiments, the protein kinase A (PKA) activity has been detected. The measurement results have shown that the ARROW-B SPR biosensors with the circulating-flow system can be applied to detect the PKA interaction quantitatively and qualitatively.