Control of biocompatibility and cellular function for osteoblasts by tunable stainless steel nanostructure

• Main Author: 謝孟哲
• Other Authors: 黃國華
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/95923510437646848136

碩士 === 國立交通大學 === 材料科學與工程學系奈米科技碩博士班 === 100 === Nanopore layers by anodization have received considerable attention in biomedical application. Previous studies have demonstrated increased osteoblast (bone-forming cell) adhesion and function on nanopore layers compared with unanodized counterparts. More recently, one study showed nanopore diameter determined cell fate. The stainless steel material is known to be much more beneficial for bone growth than others material, so there is increasing demand to explore the response of osteoblast on stainless steel with nanopore layer. For this reason, we evaluated MG63 osteoblast behavior on different diameter nanopore layers with stainless steel. Cell morphology, viability, adhesion and mineralization were evaluated. The results showed that the diameter of 40nm and 100nm provided an effective length scale for cell morphology, viability, focal adhesion, alkaline phosphatase activity, and mineralization. The cell morphology and viability showed good expression on 40nm and 100nm, best adhesion and actin filament occurred at 40nm. The mineralization rates of cells cultured on stainless steel nanopore layers increased with increasing pore diameter from 40 to 100 nm, which may be attributed to different length and nanometer-scale roughness of the nanotube layers. Our study reveals a synergistic role played by the nanotopographies in osteoblast functions and provides insight to the design of better biomedical implant surfaces.