Synthesizing and Analyzing Dinuclear Macrocyclic Complexes with the New Ligands, 12N3O-dimer and ODO2A-dimer, as Artificial Nucleases and Ribonucleases

• Main Authors: Wu, Tsung-Ta, 吳宗達
• Other Authors: Wang, Yun-Ming
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/19378615064380977830

碩士 === 國立交通大學 === 生物科技學系 === 100 === We have been interested in the design, synthesis and characterization of artificial nucleases and ribonucleases by employing macrocyclic lanthanide complexes. We use lanthanide ions to interact with ligands to form complexes. Because their high coordination number, Lewis acidity, charge density and oxidation states, they could be suitable for hydrolysis of the model compound of DNA / RNA, BNPP / HPNP. In our previous studies, we found that with more number of inner-sphere coordinated water molecules and a positive charge in the complexes, the more efficiency for hydrolysis. However, different ligands and lanthanide ions will influence the efficiency. In this research, we synthesize two new ligands, m-12N3O-dimer(1, 3-bis(1, 4, 10-triaza-7-oxacyclododec-1-ylmethyl)benzene) and m-ODO2A-dimer(1, 3-bis[4, 10-bis(carboxymethyl)- 1, 4, 10-triaza-7-oxacyclododec-1-ylmethyl]benzene) complex with transition and lanthanide ions, respectively. The result of hydrolysis studies shows that the complexes in dimer form are more effective than in monomer form with the same moiety. But the m-Eu2(ODO2A-dimer) has still lower efficiency compare to EuDO2A+. Besides hydrolysis studies, the thermodynamic characteristics are also important. These could help us to understand the whole mechanism of hydrolysis and the coordination features with lanthanide ions more accurately, and develop more efficient complexes in the future.