Development of a Immunobiosensor for Noninvasive Prostate Cancer Detection

• Main Authors: Chou, Tzongru, 周宗儒
• Other Authors: Ching, Takshing
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/83105417416345321954

碩士 === 國立暨南國際大學 === 電機工程學系 === 100 === In Taiwan, 87.3 people of per 100,000 population died of prostate cancer, and the patients’ age were over 75 years old. Under the statistics composed by Department of Health, prostate cancer for the past four consecutive years（2006 ~ 2009）to the 7th place, become the cause of cancer deaths for Taiwan. Traditional invasive testing methods are costly and time-consuming. Therefore, the purpose of this study for develop a new low-cost biosensors and were based on the impedance methods with the immune response. Measurement of biomolecules in the same time then comparison array type sensors with single type sensors, the array sensor's output impedance signal can significantly increase, but no impact on sensing sensitivity. Detection concentration and the amount of impedance change after treatment of Logarithmic, found that the amount of impedance change's detection linearity showed the best values of 2.36 MHz, R2 = 0.9953 and the slope (M value) was confirmed with the antigen's concentration increases, the amount of impedance changes will also increase. The immune biosensors in the minimum detectable concentration of 6.25 ng/mL, the array type's signal to noise ratio（SNR）increase of about 250% than single type. Overall, we can use these immune biosensors made by ourselves to conduct a objective and reliable prostate specific antigen detection.