The Effects of Endurance Training and Low-Methionine Diets on Tissue Oxidative Stress and Aging Related Protein Expression in Liver of Growing Rats

• Main Authors: Yung-YuLiang, 梁詠喻
• Other Authors: Tsang-Hai Huang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/03728870337898307301

碩士 === 國立成功大學 === 體育健康與休閒研究所 === 100 === Natural life strategies related to healthy longevity are nothing more than regular exercise and healthy diets in our daily life. Recent researches indicated that lower the content of methionine in diets could effectively delay the procedure of ageing. Since oxidative stress is well known to be associated with ageing, the low-methionine diets might be associated with decreasing oxidative stress. Moreover, whether endurance exercise training can show synergistic effects with low-methionine diets on decreasing oxidative stress or modifying effects on some negative effects caused by low-methionine diets would be valuable for further study. Purpose: To investigate the effects of endurance training and different methionine content diets on oxidative stress and aging related protein (e.g. insulin receptor, IGF-1 receptor and Akt) expression in liver. Methods: Male Sprague Dawley rats (7 weeks old, n=72) were randomly assigned to six groups, which were the 0.86％Met, 0.52％Met, 0.17％Met, 0.86％Met+EXE, 0.52％Met+EXE, and 0.17％Met+EXE groups, for each groups n=12. After an 8-week diets and exercise training intervention, animals were sacrificed under deep anesthesia. The liver tissue were collected and stored at -80℃ for further analysis. Statistical analysis were processed by using two-way ANOVA (diet×exercise) (α=.05). LSD method were used for post hoc comparison. Result: The expression of TBARS and p-Akt in liver tissue reached the significant levels in the main effect of exercise (EXEs〉non-EXEs). Significant interaction was shown in insulin receptor (post hoc comparision: 0.86%Met＋EXE group＞0.86%Met group, p= .024; 0.17%Met group＞0.52%Met group＞0.86%Met group, p〈 .001). IGF-1 receptor reached the significant level both in the main effects of diet (0.86%Mets＜0.17%Mets, p= .022) and exercise (non-EXEs＜EXEs, p〈 .001). Conclusion: Endurance exercise and low methionine diets respectively showed positive effects on regulation of oxidative stress and aging related protein expression in liver of growing rats.