Application of Blood Cells Characteristics Study by DEP Field-Flow Fractionation

• Main Authors: Chih-WeiHsu, 許智偉
• Other Authors: Tzong-Shyng Leu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/93870840225081819782

碩士 === 國立成功大學 === 航空太空工程學系碩博士班 === 100 === In this study, MEMS-fabricated interdigitated microelectrodes were used to generate the non-uniform electric fields. So the particles floating by the negative DEP force. By taking advantage of dielectrophoretic (DEP) and hydrodynamic forces, DEP Field-Flow Fractionation (FFF) technique is firstly used DEP force to levitate particles to different heights according to their different dielectric properties. Based on the parabolic velocity profile of channel flow field, particles could be separated by height or velocity difference. This study used RBCs. RBCs will affect by osmotic pressure. So the fluid must be used Isotonic solution. Isotonic solution is an important experimental parameter. If the Isotonic solution is different, they will have different electrical conductivity and dielectric. To investigate the impact of difference isotonic solution for blood cells focusing and levitation heights. And solve some experimental problems.