| Summary: | 碩士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 100 === In this research, sol-gel process was utilized to prepare ceramic coating on medical grade 316L stainless steel and different experimental parameters’ influence on physical, chemical and mechanical properties, such as annealing temperature, different solution constitute and coating layers were discussed. Coating characterizations were done by AFM and SEM (surface topography and morphology), XPS (surface chemical structures) and Nanoindentation-lateral force and scratch mode. Then human umbilical vein endothelial cells were cultured on specimens to evaluate their biocompatibility, with fluorescence staining to observe the morphology of cells, and MTT assay to evaluate the proliferation activity of cells on different materials. Finally, the proinflammatory response of cells on different specimens was assessed by enzyme-linked immunosorbent assay (ELISA) in order to see its potential for the final use in biomedical applications.
Results showed that annealing is needed to remove the solvents and additives out, and to improve mechanical properties. But its main drawback is the decreasing of surface coverage.
According to the bio-evaluation results, HUVECs didn’t show significant difference on proliferation activity on ZrO2 coatings compared to 316L stainless steel, but they do show difference on proinflammation response test. This means that ceramic coating is indeed less aggressive than stainless steel to cells, which show potential for future application in surface treatment of stainless steel as for scalpels and indwelling needle.
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