| Summary: | 碩士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 100 === Influenza is a highly number of infected individuals that make pandemic and epidemic healthcare problem. The response issue is how to in-situ correctly diagnosis and medicate that avoid overloading healthcare. With developing the technique using surface enhanced Raman scattering (SERS), the application to detection of pathogens has been evaluated. In the previous studies, Ag or Au nanorods array has been evaluated in field of virus signature or applied as the fingerprint area from the outermost surface of influenza. In the study, to create an Au-Ag multi-layered film, followed by micro/nanorod-patterning technique with Focused Ion Beam (FIB) as a SERS-active substrate improved the distinction of influenza strains.
In the study, well-ordered multi-layered Au-Ag micro/nanorod array is faced the major factor of expended EM effect with Au micro/nanorod array embedded position and thickness of Ag, and repeat the specific Au-Ag unit. A He-Ne laser with the wavelength of 633 nm, chosen R6G as a molecular probe, to estimate the enhancement factor of SERS reached to 1.74 x 107 for the Au-Ag micro/nanorod array with the surface Au 80 nm and embedded Ag 70 nm thickness. In addition, to found the limit of Au-Ag units more than 5-layer and enhancement factor bounded to 106. The factor correlated with the specific repeat Au-Ag unit is much significant than that the Au micro/nanorod array embedded position and thickness of Ag.
Base on the application study, the results of Raman spectra indicate that A/WSN/33(H1N1), A/England/12/64(H2N2), and A/Philippine/2/82(H3N2) through Au-Ag micro/nanorod array. In the Raman shift of 700-900 cm-1, it can be distinguished with each viral strain, i.e., Adenine ring (730 cm-1 for H3N2), Adenine (725 cm-1 for H2N2), and Tyrosine (854 cm-1 for H3N2 and 848 cm-1 for H1N1).
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