Specific Ring Diameter among Nano-rods fabricated by Focused Ion Beam and Used as SERS-active Substrate for Trace Detection of Molecular Probes and Various Viruses

• Main Authors: Ying-YiLin, 林穎宜
• Other Authors: Jiunn-Der Liao
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/27629026104968932581

博士 === 國立成功大學 === 材料科學及工程學系碩博士班 === 100 === Due to the discovery of surface enhanced Raman scattering (SERS), Raman spectroscopy has become a highly potential biosensing technique. To avoid the chemical pollution and complicated process, in this study we aim to utilize a simple and rapid physical method, focused ion beam (FIB), for fabrication of well-ordered nano-rods arrays on Au thin film. Through adjusting different parameters we aim to obtain the best SERS-active substrate and further to apply it for detection of trace molecular probe rhodamine 6G (R6G) and viruses. The nano-rods with different geometry, dimensions, and spacings were initially fabricated by FIB. R6G as molecular probe was chosen to estimate the effect of SERS. The results indicated higher SERS effect was appeared as dimension and spacing decreased because of more hot spot areas. In addition, we also found lighting rod effect induced by varying geometry of nano-rods also can raise electromagnetic mechanism effect to enhance signals. Furthermore, to apply for tiny trace pathogen detection, hexagon-like Au nano-rods arrays with various spacing and the “ring diameter” (DR) created by the convergence of three nano-rods as a SERS-active substrate, were fabricated. The various dimensions of target viruses (DT), and four influenza A virus strains were taken as the major factors. The results indicated that as the ratio of DR/DT was around 1, the discrimination ability for detecting the target viruses was better because of the violent enhanced lightning rod effect surrounding the target virus.