Investigate the interactome of macrophage CCAAT/enhancer binding protein delta in cotreatment of IL-1β and TGF-β

• Main Authors: Jing-ShiouHaung, 黃淨修
• Other Authors: Ju-Ming Wang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/74654887759713017190

碩士 === 國立成功大學 === 生物資訊與訊息傳遞研究所 === 100 === Epidemiologic studies suggest that chronic inflammation in aging is related to age-associated disorders, such as neurodegeneration, atherosclerosis, type II diabetes and RA, and enhanced risk of tumor formation. Over the past few decades, scientists have realized that the process of inflammation is virtually the same in different disease states. The activation of inflammatory cytokine-inducible transcription factor CCAAT/enhancer binding protein (CEBPD) has been observed in many inflammatory diseases states. However, the details of the molecular of CEBPD action in cells and pathogenesis remain largely uninvestigated. Herein, we are interested and trying to identify the interactome of CEBPD responding to treatments of IL-1β , TGF-β and IL-1β/TGF-β in macrophage by using a BIAcore/LC-MS/MS strategy. Following this idea, we purified recombinant CEBPD protein and confirmed the functional bindings of this recombinant protein or oligonucleotide-bound CEBPD on the chip. In addition, SMAD3/4, TGF-β responsive factors, were reported to interact with CEBPD and contributes to transcriptional activations of PPARG2 , COX-2 and MCP-1 genes indifferent cell types. We therefore test the bindings of CEBPD and SMAD3/4 and their consequent effect on PPARG2 , COX-2 and MCP-1 reporters in macrophages.