Production of functional antibody (IgY) in poultry eggs

• Main Authors: Jenn-FaLiou, 劉振發
• Other Authors: Lih-Ren Chen
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/08468214528339820914

博士 === 國立成功大學 === 生物科技研究所碩博士班 === 100 === Passive immunization by specific antibodies has been studied extensively against a lot of pathogens in both humans and animals. The increase in antibiotic-resistant bacteria and the demand to treat pathogens that do not respond to antibiotics, such as viral pathogens, has much research into antibodies as an alternative to antibiotics. The potential of orally administered immunoglobulin in yolk (IgY) for the prevention and treatment of a lot of pathogens have been studied for many years. This thesis was including three parts. The part Ι were to investigate the potential of IgY for oral immunotherapy with focus was conducted to characterize the specific IgY against human enterovirus type 71 (EV-71). The results of the neutralization effect of specific IgY in EV71-challenged mice demonstrate that the EV71-specific IgY, either by intraperitoneal injection or oral administration, was able to significantly reduce the morbidity and mortality in EV71 infected mice pups. Part II: were to investigate the immunization response of breeds of chickens to Escherichia coli (E. Coli) antigen and the function of specific IgY produced. The results of this specific IgY can inhibit the growth of activated E. coli at a concentration above 63 μg/mL WSF. The chicken egg-yolk antibodies in this study were able to prevent the diarrhea experimentally induced by ETEC in 21-day-old weaned piglets. Part III : Production of body weight control antibody in poultry eggs. The results of body weight control efficiency of specific IgY (anti-lipase IgY) determined using murine model indicated that this anti-lipase IgY was able to prevent obesity induced by a high-fat diet in mice. Key word: Chicken, Immunization, egg, Immunoglobulin in yolk (IgY), anti-EV71 IgY, anti-E. coli IgY, anti-lipase IgY.