The metagenomics of mycorrhiza in Phalaenopsis root

• Main Authors: Feng-YinJian, 簡鳳吟
• Other Authors: Tzen-Yuh Chiang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/19874318662446550451

碩士 === 國立成功大學 === 生命科學系碩博士班 === 100 === No matter the cut flowers or potted, Phalaenopsis sp. is the genus has economic value in the international commercial market. Phalaenopsis amabilis is the most output in Taiwan. In nature, Orchidaceae symbiosis with mycorrhizas for growth because mycorrhizas can provides nutrient for germination that Orchidaceae absent. For the requirement of the large market, Phalaenopsis sp. was cultured for breeding by the tissue culture. Compared to the wild species, cultivars may have low diversity of orchid mycorrhizas. Whether the wild Phalaenopsis amabilis or the cultivar, there were not any related researches about the mycorrhizas in the root. Hence, we explore whether the mycorrhizas exist in the KC1111 line. We were pretest for confirming the mycorrhizas existed in the root. There were 16 species belong 14 genus by fungi isolation and 26 species belong 17 genus by sequencing the ITS region. Further, for investigating the metagenomics of mycorrhizas in orchid root by Illumina. Four nucleus genes and two mitochondrial genes were as the DNA barcoding for revealing the mycorrhizal diversity. We also calculated the genetic distance between all sequences in the individual taxa for correct the bias from the dataset. It was estimated at least 230 species exist in the roots of KC1111 line. Overall, this study found the cultivars still retain the variety of mycorrhizas, and the Ceratobasidium is the most fungus in the root. The other hand, Arthrobotrys and Dactylella as the nematode trapping fungi, with the efficacy of biological control and uncultured Dendroclathra and Pachytrype were the first time found in orchid root. In addition, different DNA marker has different specificity, so we cannot detect all fungal species with a single DNA marker.