| Summary: | 碩士 === 國立中興大學 === 生醫工程研究所 === 100 === The key issues involved in tissue engineering are how to culture specific cells on a suitable scaffold and to provide a satisfactory growth factor to regulate the differentiation and proliferation of the cells. Scaffolds function as the base for cell adhesion and migration, the place for the exchange of nutrients, and to deliver and retain cells and biochemical factors. In this study, the femtosecond laser ablation technique was implemented for the fabrication of 2D pillared microvessel scaffolds of polylactic-co-glycolic acid (PLGA) and hollow 3D PLGA scaffolds.
For the 2D pillared microvessel scaffolds, PLGA scaffolds consisting of 47 μm × 80 μm pillared branches were produced. Results of cell culturing of bovine endothelial cells (BECs) demonstrate that the cells adhere well and grow to surround each branch of the proposed pillared microvessel networks. This novel scaffold facilitates implementation of the conventional cell seeding process. The progress of cell growth can be observed in vitro by optical microscopy. The problems of becoming milky or completely opaque with the conventional PLGA scaffold after cell seeding can be resolved. In this study,
For the hollow 3D PLGA scaffolds, a salt ingot which was used as a temporary frame to define the shape of the desired scaffold was fabricated by extrusion molding. The salt ingot was then immersed in a PLGA solution and allowed to be entirely enveloped by the PLGA. The femtosecond laser ablation technique was used for direct writing of the desired pattern on the PLGA layer and finally the salt ingot inside was completely dissolved in distilled deionized water to obtain a hollow 3D PLGA scaffold on which BECs were then cultured. The cell culturing results are illustrated by SEM and fluorescent images and demonstrate that the BECs could adhere well and proliferate on the branches of the hollow 3D PLGA scaffold.
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