The comparative analysis of genetic diversity in two different plants species based on different molecular marker systems

• Main Authors: Chih-Che Yang, 楊智哲
• Other Authors: 古新梅
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/92310393351110494068

碩士 === 國立中興大學 === 農藝學系所 === 100 === Begonia is one of the largest genera of the plant, containing over 1400 species. There are eighteen species in Taiwan, in which, four nature hybrid species and fourteen are endemic. In this study we used RAPD, ISSR and SRAP molecular markers to analyze genetic diversity of eighten Begonia species. All of the dendrograms constructed showed similar result, in which these Begonia species can be grouped into three clades, B. fenicis showed the distantly-related with the rest Begonia species in Taiwan. Besides, according to the similarity index, B. × taipeiensis might be the hybrid of B. formosana and B. aptera ; B. × buimontana might be the hybrid of B. palmata and B. taiwaniana. However, two hybrids in this study could not be identified their pedigree relationship. Platycerium is one of the epiphytic fern genera in Polypodiaceae. There are only eighteen species in the world which origin were suggested derived from three regions, Afro-American, Malayan- Asiatic and Javan- Australian. In this study, RAPD, ISSR and SRAP molecular markers were used to analyze genetic diversity among eighteen Platycerium species. Our result showed that Platycerium species were grouped into three clusters. In addition, P. alciorne seems to be the ancestor of P. ellisii, P. madagascariense and P. quadridichotomum. The parents of three hybrids and a cultivar were also identified successfully. In conclusion, this is the first time RAPD, ISSR and SRAP applied in the genetic diversity on these genera. All of these markers are very useful tool for genetic diversity among Begonia and Platycerium, and also discriminate the parents of hybrids.