A cell-based chip for the simultaneous measurement of cellular respiratory activity and acidification rate

• Main Authors: Shiau-An Lin, 林孝安
• Other Authors: 吳靖宙
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/58754217163555261280

碩士 === 國立中興大學 === 生物產業機電工程學系所 === 100 === Recently, the cell-based biosensors fabricated by microfabrication techniques have attracted a wide interest. The cellular respiration and acidification rate can be estimated by the change in the dissolved oxygen concentration and the pH. In this study, the microfabricated non-Clark type oxygen sensors and the electrodeposited IrOx-pH electrodes were used for the measurement of cellular respiratory and acidification, respectively. The sensitivity and thickness of IrOx electrodes electrodeposited in the range of 0.6～1.0 mA/cm2 current density (CD) doesn’t present significant difference. To Compare the effect of different electrodepositing time of 5 min (5M/0.8CD-), 10 min (10M/0.8CD-) and 15 min (15M/0.8CD-) IrOx electrodes electrodeposited at 0.8 mA/cm2 on the response time, the 90% response time were 2.2 ± 0.9 s, 7.1 ± 0.7 s and 12.8 ± 1.6 s, respectively. The result reveals that the thinner the film is, the shorter the response time becomes. However, in long-term stability test the 15M/0.8CD-depostied IrOx-pH exhibited the smallest potential drifting of 0.33 ± 0.12 mV/hr. With the utilization of on-chip IrOx reference electrode and the salt bridge microchannel, the chip-type oxygen and pH electrodes could measure simultaneously without cross talk and have great reproducibility. The diffusion-limited current could reach stable after applying reducing potential for 250 s. Moreover, the O2 and pH measurement was not affected by the convection when the flow rate was less than 20 μL/min. The chip-type cell-based biosensor can be used to estimate the effect of drugs on the cellular physiological behavior in the future, resulting in promoting the progress of drug tests on the experiments of animal and clinic diagnosis and reducing the cost.