The Mutation of Bougainvillea in vitro by Mutagents

• Main Authors: Fang-Chi Fan, 范芳綺
• Other Authors: 朱建鏞
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/91754184312828198896

碩士 === 國立中興大學 === 園藝學系所 === 100 === In this research, bougainvillea cutivars：‘Pink Pixie’、‘Pink Pixie Variegate’、‘Bella’、‘Bella M1’ and ‘Pink Panther’ were used as materials. Shoot explants were cultured on the MS medium with BA. A micropropagation system was established first from bougainvillea, then mutagents were added into the medium for inducing mutation. Shoot explants were cultured in full strength MS medium supplemented with 2 mg L-1 BA. After 8 weeks, the multiplication rate of ‘Pink Pixie’, ‘Bella’ or ‘Bella M1’ was 7.78, 8.53 or 9.42 respectively. ‘Pink Pixie Variegate’ was cultured for 8 or 10 weeks, the multiplication rate was 6.83 or 6.81. However ‘Pink Panther’ cultured for 4 weeks had a higher multiplication rate, it was 4.59. The longer culture the multiplication rate was the loss explant. The mutagent response of bougainvillea was depend on cultivars. Treat with higher concentration and longer exposure time of mutagents, the percentage of survival on lateral shoot sprouting was decreased. Explants treated with Oryzalin at 0.1%-0.3%, did not get any mutants. ‘Pink Pixie Variegate’ treated with Colchicine of 0.1％ for 1 day, proliferated a chimeric mutant ‘Pink Pixie Variegate M1’. The mutant ‘Pink Pixie Variegate M2’ were separated after several subcultures. This plant through micropropagation to establish a clone. And then following the UPOV guidelines the clone was tested its DUS. In addition, ''Pink Pixie'' treated with NaN3 of 0.25 mM for 1 day, obtained two flower-off-types mutants.