Effect of aluminum addition and mycorrhizal inoculation on growth of Pinus morrisonicola seedlings

• Main Authors: Ting-Yung Chen, 陳庭筠
• Other Authors: 顏江河
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/98559669470701960263

碩士 === 國立中興大學 === 森林學系所 === 100 === In this study, Pinus morrisonicola seedlings were planted in two kinds of soils. One of the soils called agricultural soil without aluminum toxicity. The other soils called coal mine spoils abundant exchangeable aluminum. Those seedlings were inoculated with two kinds of ectomycorrhizal fungi Pisolithus tinctorius and Scleroderma areolatum. After two months, seedlings were treated with different aluminum concentrations (0 μM、300 μM、1000 μM and 1300 μM) and different time (30 and 70 days). Finally, the biomass, plant nutrient, total soluble carbohydrates and chlorophyll were determined to evaluate the different treatments on plant growth and physiologic effects. The results showed that among all treatment, seedlings which added with 0 μM aluminum solution and inoculated with P. tinctorius had the highest biomass. The nutrient N, P, K, Ca and Mg concentration of plant tissue were decreased with increased aluminum, but the Na concentration increased when the concentration of aluminum solution was increased. Those non-inoculated seedlings contained more Na indicated that the Na was accumulated in plant tissue, but less absorption of N, P, K, Ca and Mg. On the other hand, the concentration of total soluble carbohydrate and chlorophyll were decreased when the concentration of aluminum solution were increased. Compared with agricultural soil and coal mine spoils, even though the agricultural soil was added 1300 μM aluminum solution, the exchangeable aluminum was sidnificant lower in agricultural soil than in coal mine spoils. It showed that the plant growth and physiologic effect of P. morrisonicola seedlings were worse in agricultural soil than that in coal mine spoils. Also, it was observed that seedlings growth inoculated with P. tinctorius and S. areolatum were better than those of non-inoculated. Most of the experiments, the seedlings growth inoculated with P. tinctorius were better than that of S. areolatum. Mycorrhizal inoculation could increase the tolerance of P. morrisonicola under aluminum stress. The soluble carbohydrates of P. morrisonicola inoculated with P. tinctorius were significantly lower than those non-inoculaed. In contrast, the roots dry weight, N, P, K, Ca and Mg of plant inoculated with ectomycorrhizal fungi were significantly higher than those non-inoculaed. In conclusion, seedlings inoculated with ectomycorrhizal fungi had the best performance, and those non-inoculated had the worst performance.