| Summary: | 碩士 === 國立中興大學 === 森林學系所 === 100 === Mountain Shei is located in northern Taiwan, altitude ranging from 2,100 meters to 3,886 meters thus the special climate and plants with abundant here,can be one of representative Taiwan''s mountains. The mycorrhizal (mycorrhiza) symbiotic is one of the most important mechanism for plants who wanna be adapt to the environment an, because of ectomycorrhizal which has the fruiting bodies thus can be easier to study. Traditionally, morphological analyses have been the most common method to identified ectomycorrhizal species. This purpose is using traditional morphological and molecular analyses to supporting each other.
We study how vegetation and altitude affect the ectomycorrhizal composition at the east trail of Mountant Shei in order to establish a basis ecological information for this special ecological areas. This study investigated the east trail of mountain Shei from 0 ~ 4 km, which is the trailhead to cry slope, under pine forest mixed wiyh trees of Fagaceae species, the altitude is from 2,100 to 3,000 meters. Survey along the route Every two weeks during the summer and recorded the frequency of ectomycorrhizaa arise. Then Bring back the fruiting bodies for morphological identified and strain isolated. then follow the steps for molecular identification if strain successfully isolated. Using primers ITS1F / ITS4B for PCR, amplification ITS sequences of fruiting bodies.
The results showed that there are 9 families 13 genus. 5 kinds of identification to genu and 30 identified to species, of which 15 kinds of the patterns observed were identified, 18 identified by the rDNA. There are three types of them in the both identified by morpological and molecular analyses. 2 species belonging to saprophytic bacteria were identified by molecular method. The dominance of this area are genus Suillus, the Russula is followed. Russula has the highest relative frequency, and Coltrcia has cluster. Ectomycorrhizal fungi species diversity decreased along the altitude.
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