Efficient Extraction for Chicken Skin Collagen

• Main Authors: Mei-Ting Yeh, 葉美廷
• Other Authors: Shuo-Wen Tsai
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/01290063476740168776

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 100 === Earlier, most of the raw material for collagen extraction came from bovine and swine, but since there are the epidemics situations or the religion effect, customers will hesitate in using the products made by bovine and swine. A lot of researches have been done to find a better substitute for the raw material, such as marine animals. Marine animals are an ideal material for collagen extraction, but the impurity of the extract may lead the consumers to allergy concerns. This research, in order to avoid the above-mentioned concerns, choose the chicken skin which has abundant of collagen type I as the material. Chicken is the world’s largest poultry production, but in some country, like America, chicken skin is treated as the waste. If the high value collagen could be extracted, it may increase the value of this by-product. This study proposes an efficient extraction process of chicken skin collagen. There are three steps to extract the collagen, first, the raw material is simply minced, and then ethanol is added to undergo the oil extraction, next is the alkaline treatment to remove the unnecessary proteins and finally the acid with enzyme mixture is used to extract the collagen. The oil extraction part, using 1:20 (vw) of 20% ethanol under 25℃ for 3 hr will extract about 65% of the fat. 1:20 (v/w) of 0.2 M sodium hydroxide is then added to the defatted skin and undergo the alkaline treatment for 1.5 hr, this condition may decrease the collagen loss. After the purification process, the material is soaked under 1: 10 (v/w) of 0.5 M acetate with 0.1% pepsin mixture solution for 48 hr under 25℃, the final result is the collagen solution. This method may yield 6% collagen, 85,71% recovery, and 58.63% purity. SDS-PAGE proves that using this processing method, the collagen extracted from the chicken skin is mostly the type I collagen. The procedure time compared with the previous research has been shortened to 52.5 hr, this result and the chosen raw material may enhance the competitiveness of the market.