Sequential FDG-PET/CT as a Biomarker of Response to Thalidomide in Peritoneal Gastric Cancer Dissemination in Mice

• Main Authors: Yi-Ching Chen, 陳怡靜
• Other Authors: Meei-Ling Sheu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/32626236054928093876

碩士 === 國立中興大學 === 生物醫學研究所 === 100 === Background and Purpose: A clinical positron emission tomography imaging (Postrion Emission Tomography/Computed Tomography Images) applied to the mice, a new architecture that combines the adjustment of the hardware and software on the construction of clinical positron emission tomography scanning can be applied to tumors in micedetection by the image showing the tumor cells to give the number of assessments and effective construction of a tumor suppressor effect of drug screening platform. In this paper, the use of drugs as thalidomide, thalidomide has been pointed out with anti-angiogenesis function, however, its mechanism has not yet fully clarify, in the basic experimental section, we use in vivo and in vitro tests to analyze and vascular newborn associated protein of PPAR-γ and transcription factors of the CEBP-β in the inhibition of metastasis of gastric cancer on the feasibility and mechanisms. And combined with the assessment of molecular imaging, in order to reduce the sacrifice mice in the experiment. Methods: A total of four gastric cancer cell experiment, given first of all by the three different tracer to confirm the future of drug to give way. Then scanned using positron emission tomography assessment required to give the number of tumor cells, tumor cells in mice on growth of and monitoring by image, select the best given the time of the tumor suppressor drugs, re-use continuous video monitoring drug effects. Western blot and immunohistochemical staining of the tumor in nude mice peritoneal PPAR-γ and transcription factor of the CEBP-β expression. Results: PPAR-γ and CEBP-β decreased expression in gastric cancer tissues and cells. The Salle sinus step is to influence the phosphorylation of the CEBP-β through the activation of Effect of Calpain I, reducing the expression of PPAR-γ and the CEBP-β, thereby inhibiting tumor metastasis. In addition, thalidomide effective inhibition of gastric cancer cells in peritoneal metastasis in nude mice, and in accordance with the results of image analysis. Conclusions: 1. The clinical positron emission tomography imaging as a biomarker to detecte growth and metabolism of cancer cells in mice. 2. Thalidomide effectively inhibited the metastasis of gastric cancer, this show has potential anti-cancer drugs in the treatment of gastric cancer.