Drug-induced cell apoptosis through an ornithine decarboxylase pathway

• Main Authors: Jian-Ming Haung, 黃建銘
• Other Authors: 洪慧芝
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/70878153001695299362

碩士 === 國立中興大學 === 生命科學系所 === 100 === According to the statistical data of the department of health, there about 2.5-3.5 people per hundred thousand die of leukemia per year. Therefore, the development of new anticancer drugs is a very important issue. There are many ways to resist cancer cells proliferation, induction of cell apoptosis is a very important strategy. Ornithine decarboxylase (ODC) catalyzes the first and rate-limiting step in polyamines biosynthesis. It plays an important role in cell cycle, cell growth, differentiation and apoptosis, also plays a role of tumor-promoting factor. Therefore, ornithine decarboxylase inhibition in cancer treatment and prevention of drug studies is an important research direction. The purpose of our study is using drug screening to find some drug which can inhibit ornithine decarboxylase enzyme activity. The drug screening result shows that baicalein and 1,4-naphthoquinone have a better inhibition ability. The use of two kinds of drugs to treat with the HL60 cells and found that both would lead to cell apoptosis. After drug treatment for the cells with or without overexpression of ornithine decarboxylase, ornithine decarboxylase overexpression cells can prevent apoptosis caused by baicalein and 1,4-naphthoquinone, confirmed two kinds of drugs for inducing cell apoptosis through an ornithine decarboxylase pathway.