Comparative analysis chicken genome to identify the gain & loss of protein-coding genes in evolution

• Main Authors: Ji-Hui Liu, 劉濟輝
• Other Authors: 鄭旭辰
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/89467195136001471667

碩士 === 國立中興大學 === 基因體暨生物資訊學研究所 === 100 === According to the concept of evolution, the diversity of animals originated from a common ancestry, and the more closely related species have more similarities among their genome sequences. Chicken ancestor had diverged from mammals about 310 million years ago. To study phylogenetic evolution and genetic diversity of the chicken, we used human, zebrafish and chicken as representatives to build a comparative genomics analysis. Since chicken gene annotation (~17000) is lower than zebrafish in Ensembl database (over 30000), we think there are more genes that haven’t been annotated, though with 5% chicken genome not been sequenced. Therefore we verified gene annotation of chicken through the nr and EST databases of NCBI (National Center for Biotechnology Information) to avoid excessive gene-loss analysis. We found that there are 2131 genes lost, 70 newly annotated genes and 2693 unique genes gained in evolution. We expect these results can narrow the gap between our understanding of evolution biology and molecular genomics.