| Summary: | 碩士 === 國立中興大學 === 環境工程學系所 === 100 === The objective of this study was to explore the growth and degradation capability of aerobic mixed culture in a sand column simulating a PCE- and DEC-contaminated aquifer.
The results of biostimulation study indicated that DCE removal rate could be 60 % after the operation of 57 days. With the bioaugmentation of aerobic mixed culture, DCE removal rate could be enchanced to 100 % in the upstream of a simulated aerobic aquifer after the operation of 112 days.
In microcosm study, DCE removal rate of the two indigenous reactors without and with the addation of H2O2 could be 40 % and 60 %, respectively. And DCE removal rate of the two bioaugmeanted reactors without and with the addation of H2O2 could reach to 90 %. In addition, biodegradation of DCE followed the first-order reaction.
Most of microorganisms are mainly distributed in the upstream and midstream of aerobic the simulated aquiferdue to the sufficient supply of substrates and nutrients. However, the number of microorganisms decreased along the column. After 54 days of operation, Polaromonas sp. JS666 distributed in the upstream and midstream of aerobic the simulated aquifer. After 93 days of operation, Comamonadaceae bacterium OTSz_A_293 distributed along the column. In addition, the anaerobic microorganisms, Aquitalea magnusonii and Dechloromonas sp. EMB 50 only distributed in the upstream and midstream of aerobic the simulated aquifer after the operation of 112 days. They did not distribute in the downstream of the aerobic the simulated aquifer because the DO and ORP were higher than 5mg/L and 135 mV,
respectively. Therefare, the downstream aerobic the simulated aquifer was not suitable for the growth of anaerobic microorganisms.
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