Microwave-Assisted Synthesis of Molecularly Imprinted Polymer and its Application to On-line SPE for the Determination of Bisphenol A Using Subcritical Water Chromatography

• Main Authors: Min-Ruei Huang, 黃敏瑞
• Other Authors: Jen-Fon Jen
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/288up2

碩士 === 國立中興大學 === 化學系所 === 100 === Molecularly imprinted polymers have been the important subject of intense research for several decades in both academic and industrial purposes. In this study, we investigated the microwave-assisted (MA) synthesized surface imprinted silica particles and its application to on-line molecularly imprinted solid phase extraction (MISPE) coupled with subcritical water chromatography (SWC)/ultraviolet (UV) detection for the determination of Bisphenol A (BPA) in river water. In this method, we introduced a novel microwave-assisted synthesis method for molecular imprinting of silica microspheres, and the different MA synthesis conditions were optimized including the choice of porogenic solvent, the polymerization time, and microwave power. The final synthesized microspheres or/ mirco-paraticles were characterized using field emission scanning electron microscopy (FE-SEM) and FT-IR spectroscopy, and their adsorption of imprint and non-imprint molecules was investigated. BPA was chosen as templates and good molecular imprinting was observed as evaluated using as on-line MISPE-SWC-UV. Also the different parameters that affecting the on-line MISPE-SWC-UV were thoroughly investigated and optimized, such as the samples pH, loading rate of syringe pump, and the adsorption capacity. Under the optimal conditions, the linear range were 10-1000 ng/mL for BPA in river water with RSD less than 8.4%. The detection limit was 2 ng/mL. The results proved that the proposed method was a fast, simple, low-cost and green method for the determination of BPA in river water samples.