Purification of lysozyme from fresh chicken egg white by nanofiber ion exchange membrane

• Main Authors: Jun-Yi Wu, 吳俊毅
• Other Authors: Yu-Kaung Chang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/01470519551342155174

碩士 === 明志科技大學 === 生化工程研究所 === 100 === Polyacrylonitrile nanofiber membranes were fabricated via electrospinning technique. After NaOH and HCl treatment procedures, the membrane was then used as weak ion exchange membrane. The membrane was characterized in terms of fiber diameter, porocity, specific area, pore size, ionic density, and binding capacity. In this stydy, lysozyme was chosen as a model protein. The adsorption efficiency of the modified membrane for lysozyme was assessed by the measurements of breakthrough curves in a small stirred cell system (Model Millipore 8010). The capture of lysozyme from diluted chicken egg white (CEW) with maximum working volume of 10 mL was carried out in the stirred cell system. Under the different loading concentrations, loading flow rates, rotational speed, and operating pressure. Optimal conditions (pH 9, 1 in 10 dilution of CEW, average flow rate of 3 ml/min, rotational speed of 200 rpm, and initial operating pressure of 0 psi) for the elution of lysozyme were also investigated in stirred cell experiments. It was found to be successful in achieving purification of lysozyme in a high yield of 98.2% with a purification factor of 62.9 in a single step. The experimental results of the purification process for lysozyme were scaled up to the working volume of 32.7 (Millipore Model 8050) and 70 mL (Millipore Model 8200), respectively. The experimental results showed that the scale-up processes for purification of lysozyme from CEW have the similar purification results.