Physicochemical Characterization and Biological Activities Evaluation of Curcumin Polymeric Particles

• Main Authors: Cho-Ian Tou, 杜楚欣
• Other Authors: Feng - Lin Yen
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33600391019572985830

碩士 === 高雄醫學大學 === 香粧品學系碩士班 === 100 === Curcumin (CUR) is a well known antioxidant and anti-inflammation compound which was limited to clinical applications by its poor aqueous solubility. The aim of the present study was developed a curcumin polymeric particles system using Eudragit® E100 (EE100) and polyvinyl alcohol (PVA) as carrier by solvent displacement technique in order to increase water solubility of CUR. The results demonstrated that the weight ratio of CUR: EE100: PVA at 1:2.5:2.5 (CPP255) had a fine particle size (130.96 ± 28.32 nm) with monodispersion (Polydispersity index = 0.20 ± 0.02), which was considered as the best formulation at current study. In addition, encapsulation efficiency of CPP255 was exceeded 90% which elucidated EE100 and PVA could encapsulate CUR effectively. Moreover, the result of XRD and DSC demonstrated that the crystalline CUR was transformed to amorphous state in CPP255. FTIR and UV-Vis spectra were shown an intermolecular hydrogen bond between CUR and EE100-PVA. These findings may be due to CPP255 improved the water solubility of CUR and having over 200 folds increases compared with raw CUR. Furthermore, the activities of CPP255 were evaluated by the DPPH scavenging assay, lipid peroxidation assay and UVB-induced HaCaT cell damage model. The results indicated CPP255 had better antioxidant and anti-photodamage activity than raw CUR in water. CPP255 can help CUR penetrated horny layer and retained in epidermis and dermis. Consequently, we suggest that CPP255 could be developed as cosmetic and /or medicine in further study.