Interleukin-6 Increases Promoter Binding Activity of Intestine-specific homeobox via NF-kB Signaling

• Main Authors: Ju-yi Zeng, 曾如意
• Other Authors: Shih-Hsien Hsu
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/07673351834125079885

碩士 === 高雄醫學大學 === 醫學研究所 === 100 === Chronic inflammation is often evolved into the multiple malignancies. Many literatures mentioned that interleukin-6 (IL-6) and NF-κB participate in the inflammatory and cancer processes. Intestine-specific homeobox （ISX）is a newly identified homeobox transcription factor, its expression has been linked to liver tumorigenesis. In this study, we investigated whether NF-κB can respond to IL-6 and regulate ISX gene transcription. A luciferase reporter assay should that the -260/-230 is an IL-6 responsive region. Next, we performed a nucleus/cytosol fraction analysis to examine the shuttle of IKKα and NF-κB subunits（p65, p50, p52, Rel-B, c-Rel）. It showed that only p65 shuttle can respond to IL-6 treatment. In addition, it is known that the location of activated ISX is in nucleus. We further identified potent phosphorylated site on ISX that affects the location of ISX in nucleus by immunofluoresence assay.