| Summary: | 碩士 === 高雄醫學大學 === 生物科技學系碩士班 === 100 === Phosphorylation of protein tyrosine residues regulates many cell functions and has also been proved to be involved in oncogenesis. Thus, identification of the phosphotyrosine (pTyr) proteome of cells is a very important task. Since tyrosine phosphorylation represents only around 1% of the total human phosphoproteome, the study of pTyr proteins is rather challenging. Oral squamous cell carcinoma (OSCC), the most widespread malignant neoplasm of the oral cavity, has become the fifth common cancer in Taiwanese male population. It is generally believed that oral habits, including alcohol drinking (A), betel quid chewing (B) and cigarette smoking (C), are major risk factors of oral cancer incidence. However, how these three risk factors induce oral cancer has yet to be elucidated. Since protein phosphorylation plays a critical role in oncogenic signaling pathways, in this study, we sought to identify the phosphoproteome of three oral cancer cell lines associated with the risk factors; ABC-free, B only and ABC. Currently we have established three oral cancer cell lines associated with three different risk factors. Besides, establishment of a smoking-only (C only) OSCC cell line is ongoing. We have also built up a phosphotyrosine peptides enrichment workflow coupled with mass spectrometry-based iTRAQ or label-free quantitative proteomics. In the future, we will finish quantitative phosphotyrosine proteome of oral cancer cell lines with such three combinations of risk factors and functional studies of the candidate phosphotyrosine proteins.
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