Specific Targeting of MCF-7 Breast Cancer Stem Cells

• Main Authors: Chiau-Yi Chen, 陳喬依
• Other Authors: Chao - Neng Tseng
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/87647653272361497951

碩士 === 高雄醫學大學 === 天然藥物研究所 === 100 === Stem cell plays important roles in the physiological functions of normal human. It is believed that cancer stem cell also contribute heavily to tumor formation. Cancer stem cells can also be found in established cancer cell lines, albeit in low frequency. It’s capable of self-renewal, continuous division and differentiation, thereby promote cancer proliferation. Therefore current research tends to focus on finding therapeutic agents that can eliminate cancer stem cells. In this study we tested the effect of 5-azacytidine, a DNA methyltransferase inhibitor (DNMTI). Cancer stem cells were grown in serum-free culture conditions as tumorspheres. Cytotoxicity of 5-azacytidine in MCF-7 human breast cancer stem cells survival rate was determined by MTT assay. IC50 of this drug to be approximately 10 μΜ. Cancer stem cells display a very high degree of resistance to chemotherapy and radiotherapy. This study suggests that 5-azacytidine has therapeutic potential treating in human breast cancer cell in MCF-7 by selectively targeting cancer stem cells and may provide a more effective therapeutic strategy. 5-azacytidine at this concentration significantly inhibited MCF-7 mammosphere formation, wound healing ability and MMP-9 production. Western blotting indicated 5-azacytidine induced cleavage of caspase-7 and PARP indicative of apoptosis.