Anti-inflammatory Activity, Related Mechanism And Chemical Composition Analysis Of Siegesbeckia Orientalis Extract

• Main Authors: Weng, Liwen, 翁麗雯
• Other Authors: Houng, Jeryiing
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/57900030891883167281

碩士 === 義守大學 === 生物技術與化學工程研究所 === 100 === Inflammation is a protective functionin the human body. It mainly controls infection and promotes tissue repairing. Siegesbeckia orientalis linne, a traditional Chinese medicinal herb, is usedto treat limb paralysis, rheumatism and joint pain, aching pain, lassitude, weakness, hypertension, swollen boils poison, trauma and bleeding effects. However, the literature related to the Siegesbeckia orientalis linne for scientific evidences aboutits anti-inflammatory activityis still rare. In this study, the anti-inflammatory effect of Siegesbeckia orientalis linne extract (SE) was examined in vitro and invivo.For the in vitro test, theSE showed a significant lipopolysaccharide (LPS)-induced inflammatory response on RAW 264.7 cells in the prevention and the treatment models was used. The IC50 value for the inhibition of NO formation by SE was 41.8 g/mland SE has no cytotoxicity on RAW264.7 cells. The clinical anti-inflammatory drugs L-NMMA (IC50= 21.4 g/ml) and CAY10404 (IC50= 12.2 g/ml)were used as positive control groups for comparison. Western blot analysis demonstrated that SE can effectively inhibit protein expression forinducible nitric oxide synthase(iNOS) and cyclooxygenase-2 (COX-2), and down-regulate MAPK, IBα and Akt pathways. Especially, p38 and JNK phosphorylation were the most significant effects in the MAPK pathway. From the resultsof enzyme-linked immunosorbent assay (ELISA), SE suppressed prostaglandin E2 and the pro-inflammatorycytokines IL-6 and TNF-α dose-dependently.Particularly, the effect on IL-6 was the most significant one among these three proteins.For the in vivo test, the test of λ-carrageenan-induced paw edema of ICR mouse showed that SE can effectively assuage ICR mouse paw edema swelling in a short time. Finally, gas chromatography mass spectrometry (GC-MS) were used to determine the chemical constituents of SE. Further and detailed isolation and identification for the bioactive compounds of the SE is still needed to develop it into a drug or a health food.