Screening Of Bacteriocin Producing Probiotic And Effect Of Herbal Medicines To Its Antimicrobial Ability

• Main Authors: Chao-Wei Huang, 黃兆尉
• Other Authors: Ying-Chen Lu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/32182201897956491091

碩士 === 中華醫事科技大學 === 生物醫學研究所 === 100 === In recent years, probiotics improve human health has received considerable attention. Lactic acid bacteria including lactobacilli and bifidobacteria are the most common bacterial species considered as potential probiotics . Lactic acid bacteria have an essential role in the majority of food fermentations , Its produce antimicrobial proteins and peptides called bacteriocins , It was shown to inhibit food spoilage microorganisms . The use of lactic acid bacteria fermented Chinese herbal medicine has many advantages , Such as : increase the extraction rate of Chinese Herbal Medicine、Production of new active ingredients、Reduce waste, etc . This study analyzed a total of 270 lactic acid bacteria in the laboratory culture collections, lactic acid bacteria were selected and screened for their ability to produce bacteriocin by agar well diffusion method, after the Sequence Alignment, identification of this strain is Lactobacillus gasseri. In this study, The cell density of Lactobacillus gasseri increased to 2.1×108 during 16 h, after the 24h the bacterial count started to decrease. bacteriocin production was observed at 8 hours, The best activity in inhibition of bacteriocin in 24 hours , Analysis by Fast Protein Liquid Chromatography (FPLC) , whereas the peak of bacteriocin was observed at fraction 7、8 , The activity of bacteriocin was destroyed after treatment with Proteinase K, bacteriocin remained active after 10 min at 110℃. This study used 11 different Chinese herbs medicine for fermentation, The results showed that Lactobacillus gasseri through the fermentation of Chinese herbal medicine, the growth and bacteriocin of Lactobacillus gasseri, they would have different effects, after Momordica charantia and Anemarrhena fermentation, bacteriocin produced by Lactobacillus gasseri still retained antimicrobial activity against E. coli and S. aureus, With the increase of Chinese herbs medicine concentration, Bacterial counts and the inhibitory activity also increases. The Exploration of Chinese herbs medicine to produce bacteriocin by Lactobacillus gasseri, Speculate that there are some Chinese herbal medicine will be the adsorption of bacteriocins produced by Lactobacillus gasseri, and Chinese herbal medicine will change the Lactobacillus gasseri to produce bacteriocin synthesis pathway, After the experiments, Confirmed that the Dioscorea opposita and Polygonatum sibiricum adsorption of the bacteriocin, and other Chinese herbal medicine bacteriocin reasons yet to be explored.