| Summary: | 碩士 === 中華醫事科技大學 === 醫學檢驗生物技術系碩士班 === 100 === In idiopathic pulmonary fibrosis (IPF), the most prominent change in lung architecture is the appearance of fibroblast foci in the lung interstitium. Diminution of the alveolar epithelial lining is accompanied by accumulation of fibroblast-like mesenchymal cells that secrete excessive extracellular matrix proteins such as fibrillar collagen I and III.In IPF, myofibroblasts are regarded as the main perpetrators of fibrosis as they appear to be the major source of ECM proteins such as fibrillar collagen I. Initially, it was thought that myofibroblasts develop from the differentiation of resident parenchymal fibroblasts in response to profibrotic cytokines, such as transforming growth factor-β. Transforming growth factor 1 (TGF-β1) has been identified as the main inducer of EMT in kidney and lung diseases including IPF and COPD. TGF-β1 is a wide range of cytokines that regulate growth, apoptosis, differentiation, adhesion, invasion and extracellular matrix production.TGF-β1 dysregulation of fibrosis and epithelial mesenchymal transformation related and closely linked with idiopathic pulmonary fibrosis. MMPs(Matrix metalloproteinases) are a reference of a pulmonary fibrosis. They are the main Governing the recycling of the lung tissue is an important role in the fibrosis process. MMP-9 is secreted by inflammatory cells, MMP-2 is generated by the structure of cells such as fibroblasts, epithelial cells, endometrial cells. Both tissue repair process and long-term damage and abnormal collagen accumulation related to. Andrographolide (Andro) is isolated from a traditional Chinese herbal medicine. In recent years studies.Andro can protective and choleretic, anti-inflammatory, antiviral, and increased glucose metabolism, inhibition of platelet aggregation treatment of cardiac thrombus formation, has antioxidant, anti-cancer efficacy. In the antifibrotic were few studies, we choose one of A549 cells by adding TGF-β1 causes cells to produce fibrosis, and then stimulate the 3 different doses of the Andro. Observation of MMP-2 and MMP-9, collagen and the caspase family (cytochrom-c, caspase 9, caspase 3, caspase 7,PARP) apoptosis signaling pathway, to discuss Andro reduce fibrosis lead to anti-apoptotic mechanism.The results indicate, Andro reduced collagen, cytochrom-c, caspase 9, caspase 3, caspase 7, PARP,so Andro maybe can reduce the effectiveness of pulmonary fibrosis. In the future may provide new targets of anti-pulmonary fibrosis drug development.
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