| Summary: | 碩士 === 弘光科技大學 === 化妝品科技研究所 === 100 === Armillarie mellea crude extract has been reported to show potent antioxidation and anti-lipidperoxidation activities. However, there is no report about the potential skin care effect of A. mellea crude extract. This study aimed to evaluate the posssible depigmentation effect of A. mellea crude extract. A. mellea crude extract showed potent inhibitory effects on mushroom tyrosinase (IC50=1.12 mg/ml) and B16F10 intracellular tryosinase activity. Furthermore, the A. mellea crude extract (0.075-0.5mg/ml) effectively decreased B16F10 melanin content in a dose-dependent manner. Besides, A. mellea crude extract decreased the protein content of intracellular tyrosinase-related protein-1(TRP-1). Furthermore, the possible signaling pathway involved in A. mellea crude extract depigmentation was investigated by specific regulators. Our results indicated A. mellea crude extract inhibit melanin synthesis by blocking protein kinase C (PKC) signal pathway.
Interestingly, the A. mellea crude extract also express strong antioxidant activities and significantly scavenged DPPH and ABTS radicals in a dose-dependent pattern.Additionally, the A. mellea crude extract show potent reducing power and metal ion chelating capacities, which are in accordance with previous report. To elucidate the relationship between antimelanogenic property and antioxidant activity of A. mellea crude extract, the effect of A. mellea crude extract on reactive species(RS) and reactive oxygen species (ROS) production in B16F10 cells was also investigated. However, there is no apparent corelationship between antioxidant activity and antimelanogenic effect of the crude effect. On the other hand, MTT assay data indicated that the A. mellea crude extract does not affect the proliferation of B16F10 melanoma cells, which implied that the A. mellea crude extract could be applied in the formulation of skin whitening cosmetics.
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