Inhibitory effects of Coriaria intermedia Matsum leaf extracts on herpes simplex virus type I infected human neuron cells
碩士 === 輔仁大學 === 生命科學系碩士班 === 100 === In the present study, herpes simplex virus type 1（HSV-1）infected human neuroblastoma cell line, SH-SY5Y, was set up and applied to study antiviral activities of the leaf extracts from Coriaria intermedia （CILE）. The following results were obtained:（1）The data sh...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2012
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| Online Access: | http://ndltd.ncl.edu.tw/handle/83832824526719685186 |
| Summary: | 碩士 === 輔仁大學 === 生命科學系碩士班 === 100 === In the present study, herpes simplex virus type 1（HSV-1）infected human neuroblastoma cell line, SH-SY5Y, was set up and applied to study antiviral activities of the leaf extracts from Coriaria intermedia （CILE）. The following results were obtained:（1）The data showed HSV-1 released from SH-SY5Y cells was majorly at 24 hr postinfection time（p.i.）; （2）The HSV-1 early gene such as thymidine kinase（tk）gene expressed in SH-SY5Y cells at 2 hr p.i.;（3）The HSV-1 late gene such as gC gene expressed in SH-SY5Y cells at 4 hr p.i.; （4）The results of plaque forming assay indicated CILE inhibited HSV-1 replication in SH-SY5Y cells;（5）The data from time of addition experiment indicated that CILE
blocked HSV-1 replication added at 0-24 hr p.i.;（6）The inhibitory mechanisms of CILE on HSV-1 replication were not due to cytotoxicity;（7）CILE did not affect HSV-1 released from SH-SY5Y cells;（8）The results from polymerase chain reaction（PCR）showed that CILE did not decrease HSV-1 DNA synthesis;（ 9 ）The data from reverse transcription-PCR（RT-PCR）demonstrated that CILE did not block viral late gene expression;（10）CILE inhibited the levels of HSV-1 late structure proteins;（11）CILE blocked the adsorption and entry of HSV-1; （12) CILE damaged the HSV-1 structure. In this study, I set up the model of HSV-1 infected SH-SY5Y cells. I proved that CILE inhibited HSV-1 replication in SH-SY5Y cells by blocking of viral adsorption and entry, decrease of late proteins synthesis, and interruption of HSV-1 structure.
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