Extraction of Triterpenoids from Antrodia cinnamomea Fruiting Bodies Grown on China Fir and Optimization of Growth Conditions

• Main Authors: Chan, Yian, 陳頤安
• Other Authors: Ko, Wenching
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/70440476106715382199

碩士 === 大葉大學 === 生物產業科技學系 === 100 === This work focuses primarily on the growth and development of Antrodia cinnamomea fruiting bodies on China fir. We also analyzed the triterpenoids isolated from the fruiting bodies of A. cinnamomea and investigated their ability to scavenge DPPH by HPLC mapping analysis to identify the differences in the compositions of the fruiting bodies. Previous studies have shown that fewer fruiting bodies grow upon culture with China fir, yielding a triterpenoid level of 3.25% and a DPPH scavenging power of 67.25% (0.01 mg/ml), than upon culture with A. cinnamomea, yielding a triterpenoid level of 4.31% and a DPPH scavenging power of 72.31% (0.01 mg/ml). HPLC mapping analysis also revealed a number of differences in their composition. We used ultrasound techniques to efficiently extract and purify triterpenoids from fruiting bodies growing on China fir and found that different alcohol concentrations, temperatures, and liquid-to-solid ratios determine the efficiency of the triterpenoid extraction. Using a reverse methodology, we achieved the most efficient conditions using an alcohol concentration of 74.38%, a temperature of 60.41°C, and a liquid-to-solid ratio of 1 g/104.81 ml. We used a molecular template technique to effectively separate different triterpenoid compounds within different extractions. Separation rates of 68.47% and 85.31% were achieved using squalene and 18-β-glycyrrhetinic acid as the molecular templates, respectively. Our results show that A. cinnamomea fruiting bodies can be cultured artificially on China fir. Useful components can be effectively separated using the molecular template technique, thus enhancing the productivity and pharmaceutical value of A. cinnamomea fruiting bodies.