Evaluation Bioactivity of Extracellular Low-polar Components from Coriolus versicolor LH1

• Main Authors: Tsai, Kunlin, 蔡坤霖
• Other Authors: Yang, Powen
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/92615185481996335727

碩士 === 大葉大學 === 生物產業科技學系 === 100 === Coriolus versicolor is a valuable medicinal fungi, it has been used very long time ago in the east. It contains polysaccharides, saponins, triterpenes, adenosines. Their physiological activities are strengthening the immune system, lowering blood pressure, lowering blood sugar, lowering blood pressure. The material is the supernatant after alcohol precipitation of C. versicolor LH1 extracellular fermentation, take the high-molecular (12-16 kDa). It is further separated by DEAE-Sepharose CL-6B column and 8 fractions were collected, analyzed composition and activity separately. The results showed that the fraction 4 has the best DPPH scavenging activity and ability to inhibit α-glucosidase. The fractions were purified using resin HP 20 packing column, and 7 fractions were collected, analyzed composition and activity separately, and calculated their molecular weights were by using HPLC and RI detection. Then take three fractions, which has better α-glucosidase inhibition activity, were filtrated by Sephadex G-50 gel chromatography, analyzed their functional groups by using FTIR, and analyzing their sugar composition of polysaccharide fractions. The results showed that the supernatant after alcohol precipitation of C. versicolor LH1 extracellular fermentation has great antioxidation and α-glucosidase inhibition activities, It is expected to use for health food, that the C. versicolor can be used more completely.