Effects of Media and Culture Conditions on Production of Bioactive Ingredients by Wolfiporia cocos and Lactobacillus acidophilus Co-culture

• Main Authors: Lu Chih Hao, 呂志豪
• Other Authors: Tai-Hao Hsu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/53469368632521797318

碩士 === 大葉大學 === 生物產業科技學系 === 100 === Many studies had reported that medicinal fungi and lactic acid bacteria possess a number of different bioactive properties, including anti-oxidant、immunomodulatory, and anti-tumor bioactive functions. For the two species, co-culture systems using fermentation studies were conducted less frequently. Therefore, this study was conducted employing a co-culture system of the medicinal mushroom - Wolfiporia cocos and lactic acid bacteria- Lactobacillus acidophilus. Natural carbon and nitrogen sources were used instead of a half-compound medium. Effects of culture conditions on the production of bio-active ingredients in a 5L stirred tank fermenter(PTF). Further, to assess of antibacterial activity and antioxidant activity. The results: Optimal medium carbon source 4% food grade- sucrose and nitrogen source 1% peanut powder. Add to 4% of Lactobacillus acidophilus co-cultured, the best production by polysaccharides and triterpenoid. In 5L fermenter culture conditions of optimum culture growth conditions, at 25℃, 150 rpm, 1vvm, initial pH=5.5, that was the most suitable conditions to bioactive ingredients with the production. Under the same conditions (1vvm, 25℃, 150rpm, initial pH5.5), employing single strain cultures and double strain co-cultures it was found that there was significantly greater extracellular polysaccharide (EPS), intracellular polysaccharide (IPS), triterpenoid, ergosterol and polyphenol improvement in the double strain co-culture than the single strain culture (10.7mg/mL, 1.5mg/mL, 0.7mg/mL, 0.6mg/mL, 2.73 mg/g, 31.08 mg/g, respectively).The antibacterial activity results: Six strains of pathogenic, liquid antibacterial test, including Staphylococcus aureus and Escherichia coli were better, the zone of inhibition were 8.5mm and 7.4mm. Minimum inhibitory concentration (MIC) of the 10% test, including Staphylococcus aureus and Bacillus cereus were better.The antibacterial stability test, including Staphylococcus aureus and Escherichia coli were better.