| Summary: | 碩士 === 中原大學 === 機械工程研究所 === 100 === The microfluidic lab-on-a-chip uses easy and convenient ways to accomplish the needed functions to proceed biomedical testing with micro reagent and chemical adjustment. In order to achieve integration for advantages of relevant chips, it adopts professional methods in different fields to design structure of chips to accomplish a complete process for biological test. This kind of microfluidic lab-on-a-chip is assembled by many different forms. This research is trying to use one of physical property of fluid dynamics which is incompatibility property of oil and water to form encapsulated droplet from two different liquid. When the oil encapsulates the reagent, it can produce a group of droplet to reach a clean and no cross-contamination between reagents. With other liquid properties such as flow rate etc., it can precisely control amount of encapsulated reagent in the droplet. The method for producing droplet is a relatively simple mechanical design to achieve action of encapsulation. The advantages of droplet system in this research are micro reagent, low cost, accurate merging and functions of adjustment for reagent. It can generate arrayed serial droplet which is helpful for simplifying biomedical testing that is time and work consuming to complete a large number of testing. The bonding forces from metallic covalent bond between nickel and cobalt and biotin have testing functions. When applying metallic coatings of nickel and cobalt on the micro-droplet lab-on-a-chip, it can simplify biological testing procedures and produce massively. With basic concept of micro-droplet, it can simplify inextricable protein testing. In brief, droplet can be thought as a micro test tube, and in droplet test tube, it has features such as clean and easy to operate etc. It needs strictly control the dose in protein experiments and it will bring different result in experiments if there is a slight change. Especially in biomedicine, it needs more accurately control for collocating doses that it won’t endanger other biological parts or bring other unexpected results. In addition, micro-droplet can deal with this kind of micro control. Through merging droplets and design of machinery to complete mixing process, then with different surface treatment of NC chips, it can deal with after forming droplet testing more easily, and it can integrate different technologies together to become a microfluidic lab-on-a-chip. Technologies which used in this research are simple and easy obtained technologies.
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