Effects of LED Light Irradiation on the Secretion of TGF-β1 from the Burns of Hyperglycemic Rats

• Main Authors: Yi-Ting Liu, 劉益廷
• Other Authors: Mu-Zon Wu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/50492357573705326247

碩士 === 中原大學 === 生物醫學工程研究所 === 100 === The aim of this study was to determine effects of LED light irradiation on the secretion of TGF-β1 from the burns of hyperglycemic rats. We used Wistar rats for our experimental animals. All rats were devided into six groups. Hypergiycemic rats were induced with a single intraperitoneal dose of alloxan monohydrate (80~100 mg/kg) after an 18 hour overnight fast.The blood glucose was tested on 4 and 8 days after induced and blood glucose level more than 300 mg/dL were selected for the further study. The burned wound was created on the back of rats using a metal rod preheated to 100~110℃. Each wound size was 1×1cm2. The LED was given with these parameters: 630 nm of wavelength, 14 mW/cm2 of power density, 4 J/cm2 of energy density for 280 seconds, and once for two days.The wound healing were evaluated by histological analysis and the secretion of TGF-β1. According to the results of cell count, in the normal rats, LED could make the maxima of macrophage and fibroblast appear early two days. In the hypergiycemic rats, LED made the maxima of macrophage appear early two days, and the maxima of fibroblast appear early four days. In the part of angiogenesis, our result showed that LED could accelerate the rate of angiogenesis and made the maxima of angiogenesis appear early two days. The result of TGF-β1assay showed that the LED seem to have no significant influence on normal rats. In hyperglycemic rats, LED could reduce the concentration of TGF-β1 in the last phase of wound healing. Our result showed that LED could make the maxima of macrophage, fibroblast and vessel early appeared, and reduce the concentration of TGF-β1in hyperglycemic rats. In conclusion, LED could make the healing time of normal rats early two days, and reduce the concentration of TGF-β1 on the last phase of wound healing in hyperglycemic rats to promote wound healing.