The attachment and differentiation of neural stem cell on silica nano fiber

• Main Authors: Po-Zen Fan-Jen, 范姜柏人
• Other Authors: Ting-Yu Chin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/ppkz2j

碩士 === 中原大學 === 生物科技研究所 === 100 === Electrospinning technique using organic and inorganic materials into three dimensional fibrous structures was proven to be useful. In the past, pointed out that the electrospinning technique using organic and inorganic materials into three dimensional spinning fiber structures is conducive to cell attachment and growth. Silica is a low toxicity, stability of inorganic compounds. Our research aims to find suitable adhesion and differentiation of neural stem cells (NSCs) in silica nanofiber materials. NSCs isolated from 13.5 days of embryonic brains were propagated as neurospheres and culture under organic and inorganic nanomaterial conditions. Silica or Silica conjugated amino groups (Silica-AP) were used as substrates to culture NSCs to examine the effects of these biomaterials on the properties of neural stem/progenitor cells (NSPCs) in the presence of dibutyryl cyclic AMP (dbcAMP) in growth factors-free medium. To test whether the NSCs-derived neurons could express neuronal specific properties, the differentiated cells are characterized by morphological observation, immunostaining and QRT-PCR for neuronal specific proteins. We use the microscopic observation of neural stem cells in different types of nano-materials growth situation. Then we used QRT-PCR identification the mRNA expression level of neuron-specific enolase (NSE) which is neuron labeled protein. Using SEM to identified the morphalogy of neural stem cell. Present results indicate that the silica conjugated amino groups help the differentiation of neural stem cells.