| Summary: | 碩士 === 中原大學 === 生物科技研究所 === 100 === Diabetes mellitus (DM) is a chronic disease characterized by elevated levels of sugar in the blood. It's one of the prevalent diseases of modern times. DM not only list on the "top ten causes of death" in Taiwan, but also the prevelence rate of it increasing, accompanied with a decreasing average age of incidence annually. In addition, DM would also induce other complication diseases, for example coronary heart disease, renal disease and hypertention. Apparently, DM is a significant disease for people in Taiwan. Our previous study showed that water extract of longan flower (LF) could ameliorate the multiple symptoms in metabolic syndrome (MS). MS frequently occurs in people with DM. Therefore, the aim of this study was to further examine if the longan flower could improve DM and the complication of DM.
The first part of this study is an animal study. Nicotinamide and streptozotocin-induced type 2 diabetic Sprague-Dawley rats were used as a animal model. Rats were administered with water extracts of longan flower (LF), ethyl acetate fractions of LF (EA) and water layer fractions of LF (W) respectly to evaluate the hypoglycemic effect of LF and its different fractions. Following, the assay of glucose/dicarbonyl compound induced formation of advanced glycation end products were used to assess the preventing effects of LF and its different fractions on diabetic complication.
Results of in vivo study showed that EA fraction could improve the hyperglycemia and hypertriglyceridemia in diabetes rats. And also, LF and EA fraction ameliorated the glucose intolerance which is considered to be a stage in the development of type 2 DM. Results of in vitro study showed that LF could inhibit the formation of advanced glycation end product, and the most effective fraction was EA fraction.
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