| Summary: | 碩士 === 中原大學 === 生物科技研究所 === 100 === ABSTRACT
The worldwide rapidly growing prevalence of obesity has resulted in a significant increase in the metabolic syndrome (MS), diabetes mellitus and associated cardiovascular disease. Lipid-lowering therapies have been investigated to improve and prevent pathophysiologic conditions such as dyslipidemia, diabetes, and hypertension. Therefore, this study aims to evaluate the benefits from using the supplementation of various healthy foods on mechanism of lipid-lowering activity in high fructose diet-induced metabolic syndrome rats and in mice liver cell line (FL83B) under oleic acid condition.
This study was carried out into two parts. Firstly, male Sprague- Dawlay (SD) rats were be randomly divided into 3 groups: blank group (B): fed with normal diet; control group (C): fed with high fructose diet alone; treatment group (T): fed with high fructose diet plus 0.5% roselle, 0.4% chlorella and 0.1% bitter melon extract. The study lasted for 10 weeks. Risk factors of MS including blood pressure, body fat mass, plasma glucose, triglycerides (TG) and total cholesterol (TC) were measured at the end of 10 weeks dietary manipulation.
The data demonstrated that metabolic disorders such as higher abdominal fat mass, blood pressure, blood glucose and plasma lipid levels were shown in group C compared to group B. These results indicated that high fructose diet could cause a cluster of disorders in MS. In order to further evaluated the possible mechanism of high fructose diet associated with dyslipedimia, we found that the rats in group C showed a decrease phosphorylation activity of acetyl CoA carboxylase (ACC) protein.
Compared to group C, the significant improvement of systolic blood pressure, fasting plasma glucose and liver lipid contents were shown in group T. And the decrease of lipid profile in liver might result from the increased phosphorylation of AMP-activated protein kinase (AMPK) and ACC.
Moreover, this study also investigated the possible mechanism of lipid-lowering activity of healthy food in a oleic acid (OA) induced lipid accumulation model in FL83B hepatocyte cells. Protein content of AMPK, p- AMPK and ACC, p-ACC were measured by Western blot.
Treatment with oleic acid induced a significant lipid accumulation in hepatocyte cells which is suppressed by supplementation with roselle, chlorella and bitter melon respectively . In western blot assay showed these three healthy food slightly increased phosphorylation activity of AMPK and ACC but without difference with OA treatment group.
According to presents findings, we suggested that supplementation of combination of combination of roselle, chlorella, and bitter melon seems to be a good measure to obesity and improve dyslipidemia.
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