| Summary: | 碩士 === 中原大學 === 土木工程研究所 === 100 === Dioxin and Furan (simply as Dioxins) are persistent organic pollutants which are highly stable and less decomposed. Dioxins are bioaccumulated and biomagnified. They are easily accumulated in the food chain and finally enter the human bodies to result in health impact. Therefore, fast screen these dioxins-polluted foods is a hot issue.
HRGC-HRMS is the main chemical analysis method for dioxins. The dioxin pollution events are always breaking news and require instant response to provide valuable information and data within a short time. Chemical analysis method is though accurate, but time-consuming and expensive, not suitable for largescale dioxin surveillance. Therefore, Chemically Activated LUciferase eXpression bioassay was progressively developed to apply to the fast prescreening.
In this study, three extracted solvent: toluene, n-hexane/methylene chloride and n-hexane; two extracted method: soxhlet extraction and ultrasonic extraction and two clean up procedure: direct concentration and column clean up were applied to pretreat ash samples. Ad-DRE-Luc/H4IIE cells based DRE-Luciferase bioassay was used for analysis. The results showed that there was good correlation between DRE-Luciferase bioassay and chemical analysis for ultrasonic extracted samples following column clean up procedure. The correlation coefficient is 0.873 for all three solvent samples. Toluene (R2=0.984) as the extraction solvent had the best outcome, followed by n-hexane/Methylene chloride (R2=0.929) and n-hexane (R2=0.883). For soxhlet extraction column clean up process, n-hexane/Methylene chloride and the toluene solvent extracts also had good correlation, R2=0. 898 and R2=0.887, respectively. The low correlation coefficient of DRE-Luciferase bioassay and chemical analysis method for direct concentration samples between ultrasonic extraction and soxhlet extraction was only 0.010 and -0.026.
The preliminary result shows that DRE-Luciferase bioassay following the column clean up pretreatment provided a better detection results for ash samples.
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