Effects of Coptis chinensis extracts on the human lung adenocarcinoma cell line A549

• Main Authors: Yi-Ming Lai, 賴奕銘
• Other Authors: Rong-Fong Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/81101813654422993511

碩士 === 中臺科技大學 === 醫學生物科技研究所 === 100 === The mortality of lung cancer leads the maligancies around the world, it takes place more than one million cases every year. Traditionally, cancer is treated through surgery and/or combined with chemotherapy or radiotherapy. Nevertheless, chemotherapy and radiotherapy often result in side effects or drug-resistance. Recently, natural compounds are widely used for cancer therapy. Treatment of cancer cells with natural compounds usually results in cell cycle arrest and/or apoptosis. Huanglian (Coptis chinensis), a widely used herb as a traditional Chinese medicine, has been shown to possess function of anti-microorganisms, alleviating hyperglycemia, increasing cardiac contraction, and anti-carcinogenesis. Recent studies have shown that crude extracts of huanglian and a major component of it, berberine, reveal anticancer activities, including inhibition of cell growth through cell cycle arrest and/or apoptosis. In this study, effects of crude extracts from C. chinensis on A549 cells derived from lung cancer were investigated. The cell number of A549 decreased 2 days after treated with the ethanol extract of C. chinensis, with IC50 of about 20