Immunomodulatory and Anti-inflammatory effects of Agaricus blazei Polysaccharides

• Main Authors: Chin-chun Lin, 林錦均
• Other Authors: Kin-mu Lee
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/82108764091277796751

碩士 === 中臺科技大學 === 醫學生物科技研究所 === 100 === Agaricusl blazei Murill has been traditionally used as a health food source in Brazil for the prevention of cancer, diabetes, hyperlipidaemia, arteriosclerosis and chronic hepatitis. In this study, BALB/c mice were fed with A. blazei polysaccharides at different time points to investigate the anti-inflammatory action and the immunomodulatory effect. When mice were administrated A. blazei polysaccharides for a period of time and then injected LPS into mice, The Trem-1HI expression on macrophages is inhibited. The expressions of CD40HI and CD8αHI markers on DCs increased apparently. However CD8HI maker on macrophages decreased significantly, this result may regulated by the activation of CTL-4 and ICOS from T cells. In addition, we use real-time PCR to detect Trem-1、TNF-α、IFN-γ、iNOS、IL-1β、IL-6、IL-12、CCL2 and PGE2 mRNA cytokine inhibited obviously. The results mice demonstrated A. blazei polysaccharides possesses effects on both immunomodulation and anti-inflammation. These makers may serve as targets for further understanding the molecular basis and efficacy of A. blazei polysaccharides and other Chinese herbal medicines in the future.