Application feasibility of optical micro-manipulation to single cells for clinical studies.

• Main Authors: Min-hui Huang, 黃閔輝
• Other Authors: Chih-Lang Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/09209105204762355141

碩士 === 中臺科技大學 === 醫學工程暨材料研究所 === 100 === In this study, the manipulation characteristics of laser tweezers exerted at micro-sized particles are applied for investigating the feasibilities of clinical studies and examinations of single cells by trapping and dragging the experimental subjects: amniotic fluid cells (20 µm) and staphylococcus (1 µm). The experiments include (1) to collect the amniotic fluid cells which are put in the microfluidic chip for the cells separation: to investigate the practical operation parameters, i.e. laser power and dragging speed, in order to harmony with the real dragging route in the micro-channels; (2) to measure the exerted forces of laser tweezers which are at the different resistances of staphylococcus aurous in order to distinguish the strains of VSSA and VISA. The above experimental achievements are expected to contribute on the development of the culture and the quantitative analysis of whole genome of single cell, and a fast examination of staphylococcus aurous for medical doctor to prescribe for treatment.